Cannabis seed removal

Selective Extraction of Cannabinoid Compounds from Cannabis Seed Using Pressurized Hot Water Extraction

Phytochemicals of Cannabis sativa mainly for the use in the different industries are that of delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Pressurized hot water extraction (PHWE) is seen as an efficient, fast, green extraction technique for the removal of polar and semi-polar compounds from plant materials. The PHWE technique was applied to extract cannabinoid compounds from Cannabis sativa seed. Response surface methodology was used to investigate the influence of extraction time (5-60 min), extraction temperature (50-200 °C) and collector vessel temperature (25-200 °C) on the recovery of delta-9-tetrahydrocannabinol (THC), cannabinol (CBN), cannabidiol (CBD), cannabichromene (CBG) and cannabigerol (CBC) from Cannabis sativa seed by PHWE. The identification and semi quantification of cannabinoid compounds were determined using GCXGC-TOFMS. The results obtained from different extractions show that the amount of THC and CBN was drastically decreasing in the liquid extract when the temperature rose from 140 to 160 °C in the extraction cell and the collector’s vessel. The optimal conditions to extract more CBD, CBC, and CBG than THC and CBN were set at 150 °C, 160 °C and 45 min as extraction temperature, the temperature at collector vessel, and the extraction time, respectively. At this condition, the predicted and experimental ratio of THCt (THC + CBN)/CBDt (CBD + CBC+ CBG) was found to be 0.17 and 0.18, respectively. Therefore, PHWE can be seen as an alternative to the classic extraction approach as the efficiency is higher and it is environmentally friendly.

Keywords: cannabinoid compounds; pressurized hot water extraction; response surface methodology.

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Conflict of interest statement

The authors declare no conflict of interest.

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Pressurized hot water extraction setup…

Pressurized hot water extraction setup with showing trap solution and oil heat bath…

I have an indoor growroom and in my recent harvest I found seeds in the buds, but I’m sure there are no male plants in the room. I’ve heard that light leakage can cause plants to become hermaphrodites. Is this true, and if so, do you have any tips for avoiding this?

Cannabis plants are monecious. This means they have the ability to be either male or female. Or in the case of hermaphroditism, they can be both. The reason to make sure there are no males or hermaphrodites in your garden is because male flowers make pollen. When pollen touches the white hairs on a flower, it makes a seed, and seeded weed gives you headaches. Even though there are reasons in nature hermaphroditism could be important, such as continuing the species in case there is no male present, hermaphroditism is generally a bad thing when talking about cannabis plants.

Light poisoning is the most common cause for a normal plant to hermaphrodite.

Light poisoning refers to the flowering night cycle of a plant being unnaturally interrupted with light. The best way to prevent this is to close yourself inside your darkened room during the daylight, and then after allowing a few minutes for your eyes to adjust to the dark, check for any light leaks from covered windows, door jams, etc. Also cover all timer and appliance lights with tape.

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Negative stressors can combine with small interruptions of the light cycle to cause hermaphroditism, especially with less-stable, clone-only hybridized strains. When the night cycle is abnormally interrupted, it sends a mixed hormonal signal to the plant. This can cause a full female plant to throw some male flowers. Male flowers are easy to identify, especially when side by side with female flowers. Male flowers look like small bunches of bananas, which will take a week or two to swell before they burst and release their pollen.

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Finding a hermaphrodite in your growroom can happen at any stage of the flowering cycle and is indicated by the presence of male flowers growing on the same plant as female flowers. As with all species in nature this can occur in varying degrees. A plant can become slightly or majorly hermaphroditic. In cases where singular male flowers are found between the branch and stalk nodes, you should be diligently removing them as they grow. You must re-inspect the plant top to bottom every few days to be sure pollination and seeding doesn’t occur. If you find male flowers (anthers) actually growing from within the female flowers (buds) the situation is a little more dire. You can still remove all the male anatomy as it appears, but it will be harder to find and much more prevalent. This is a horrible discovery that leads to a tough decision: Should you let the plant live and risk the whole crop being ruined by seeds?

In either case, once hermaphroditism has compromised the safety and purity of your sensimilla, the plant should not be propagated further. Remember, once a hermy, always a hermy. The plant pictured here is in the tenth and what should have been the final week of ripening, but a timer failed and one light stayed on continuously for almost two weeks, causing this vegetative regrowth. Because the light was continuous, the plant made no pollen. This method of re-vegging can be used to save a flowering plant you have no copies of, but be careful, as this may cause some strains to hermaphrodite.

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Purposefully causing a plant to hermaphrodite is called selfing. Gibberellic acid or colloidal silver is typically sprayed onto the female plant. This technique is used to make feminized seeds and uses the plant’s ability to be both male and female to force a female plant to produce male flowers. The pollen contained in these male flowers can only produce female seeds. Just keep in mind that feminized plants should not be used for breeding, as they were produced without a true male, making them genetically inferior.